Burkholderia cenocepacia J2315, BCAL3240 (wzt2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAL3240
NCBI Locus TagQU43_RS52910
Name
wzt2
Synonym: cpxA
Replicon chromosome 1
Genomic location 3547504 - 3548157 (- strand)

Cross-References

RefSeq WP_006484936.1
GI 206561584
Entrez 6933098
GI 206561584
INSDC CAR53566.1
NCBI Locus Tag QU43_RS52910
NCBI Old Locus Tag BCAL3240
RefSeq WP_006484936.1,GeneID
UniParc UPI00017B98CC
UniProtKB Acc B4EDF8
UniProtKB ID B4EDF8_BURCJ
UniRef100 UniRef100_A0A0D1BIA4
UniRef50 UniRef50_A3N2N3
UniRef90 UniRef90_A0A0D1BIA4

Product

Feature Type CDS
Coding Frame 1
Product Name
capsule polysaccharide export ATP-binding protein, CpxA
Product Name Confidence: Class 2
Synonyms putative capsular polysaccharide transporter ATP-binding protein
Evidence for Translation
Charge (pH 7) 2.80
Kyte-Doolittle Hydrophobicity Value -0.215
Molecular Weight (kDa) 24424.8
Isoelectric Point (pI) 8.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0436
Category Type I O-polysaccharide
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Notes
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080
Source 2
Database BGD
Category Exopolysaccharide
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes cpxA/wzt2 mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG006217 (435 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL3240
Search term: wzt2

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 6 [Source:HGNC Symbol;Acc:HGNC:36]
E-value: 6.5e-13
Percent Identity: 25.7
Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 9 [Source:HGNC Symbol;Acc:HGNC:39]
E-value: 6.5e-13
Percent Identity: 25.6
Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 9 [Source:HGNC Symbol;Acc:HGNC:39]
E-value: 6.5e-13
Percent Identity: 25.6

References

Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo.
Hunt TA, Kooi C, Sokol PA, Valvano MA
Infect Immun 2004 Jul;72(7):4010-22
PubMed ID: 15213146