Burkholderia cenocepacia J2315, BCAL3119 (wbiI)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
Locus Tag
NCBI Locus TagQU43_RS52325
Replicon chromosome 1
Genomic location 3409126 - 3411009 (- strand)
CommentBelongs to the O antigen biosynthesis gene cluster


RefSeq WP_006485114.1
GI 206561465
Entrez 6934257
GI 206561465
INSDC CAR53443.1
NCBI Locus Tag QU43_RS52325
NCBI Old Locus Tag BCAL3119
RefSeq WP_006485114.1,GeneID
UniParc UPI00017B9956
UniProtKB Acc B4ECE0
UniRef100 UniRef100_B4ECE0
UniRef50 UniRef50_G2J9Y1
UniRef90 UniRef90_B4ECE0


Feature Type CDS
Coding Frame 1
Product Name
nucleotide sugar epimerase/dehydratase
Product Name Confidence: Class 1
Synonyms WbiI
Evidence for Translation
Charge (pH 7) 6.50
Kyte-Doolittle Hydrophobicity Value 0.171
Molecular Weight (kDa) 69230.0
Isoelectric Point (pI) 8.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Siderophore biosynthesis and uptake (ornibactin)
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes wbiI mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 464 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000742 (649 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BCAL3119
Search term: wbiI

Human Homologs


Correlation of wbiI genotype, serotype, and isolate source within species of the Burkholderia cepacia complex.
Vinion-Dubiel AD, Spilker T, Dean CR, Monteil H, LiPuma JJ, Goldberg JB
J Clin Microbiol 2004 Sep;42(9):4121-6
PubMed ID: 15364998
Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis.
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA
J Bacteriol 2005 Feb;187(4):1324-33
PubMed ID: 15687196