Burkholderia thailandensis E264; ATCC 700388, BTH_II0256 (ppkA-6)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia thailandensis E264; ATCC 700388
Locus Tag
NCBI Locus TagBTH_RS01330
Replicon chromosome II
Genomic location 313510 - 316143 (+ strand)
Transposon Mutants 2 transposon mutants in E264; ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-6 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170).


RefSeq YP_438458.1
GI 83716861
Entrez 3845191
GI 83716861
INSDC AIP28624.1
NCBI Locus Tag BTH_RS01330
NCBI Old Locus Tag BTH_II0256
RefSeq WP_009895014.1
UniParc UPI0000667580
UniProtKB Acc Q2T8P1
UniRef100 UniRef100_Q2T8P1
UniRef50 UniRef50_A1UYY9
UniRef90 UniRef90_Q2T8P1


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system-associated protein TagE-6
Product Name Confidence: Class 2
Synonyms serine/threonine protein kinase
Evidence for Translation
Charge (pH 7) -3.17
Kyte-Doolittle Hydrophobicity Value -0.418
Molecular Weight (kDa) 90706.8
Isoelectric Point (pI) 6.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 400 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG016589 (492 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0256
Search term: ppkA-6

Human Homologs

Ensembl 86, assembly GRCh38.p7
p21 (RAC1) activated kinase 2 [Source:HGNC Symbol;Acc:HGNC:8591]
E-value: 1.3e-13
Percent Identity: 26.3


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD.
PLoS Pathog. 2010 Aug 26;6(8)
PubMed ID: 20865170
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS.
Microbiology. 2007 Aug;153(Pt 8):2689-99.
PubMed ID: 17660433
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA(1), Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D.
Mol Microbiol. 2007 Jun;64(6):1466-85.
PubMed ID: 17555434