Burkholderia cenocepacia J2315, BCAM0187
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Ontology
| Ontology | Accession | Term | GO Evidence | Evidence Ontology (ECO) Code | Reference | Comments |
|---|---|---|---|---|---|---|
| Molecular Function | GO:0003852 | 2-isopropylmalate synthase activity |
Inferred from Sequence Model
Term mapped from: InterPro:PF08502
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0019752 | carboxylic acid metabolic process |
Inferred from Sequence Model
Term mapped from: InterPro:PS00815
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0046912 | transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer |
Inferred from Sequence Model
Term mapped from: InterPro:PS00815
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0003824 | catalytic activity |
Inferred from Sequence Model
Term mapped from: InterPro:PF00682
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0009098 | leucine biosynthetic process |
Inferred from Sequence Model
Term mapped from: InterPro:PF08502
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
Pathways
| Database | Xref | Pathway | Version | Evidence | PMID |
|---|---|---|---|---|---|
| MetaCyc | 3-methylbutanol biosynthesis (engineered) | InterPro 5.36-75.0 |
ECO:0000259
match to InterPro signature evidence used in automatic assertion |
||
| KEGG | bcj01210 | 2-Oxocarboxylic acid metabolism | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
|
| KEGG (InterPro) | 00290 | Valine, leucine and isoleucine biosynthesis | InterPro 5.36-75.0 |
ECO:0000259
match to InterPro signature evidence used in automatic assertion |
|
| KEGG | bcj01110 | Biosynthesis of secondary metabolites | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
|
| KEGG | bcj00620 | Pyruvate metabolism | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
|
| KEGG | bcj00290 | Valine, leucine and isoleucine biosynthesis | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
|
| KEGG | bcj01100 | Metabolic pathways | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
|
| KEGG (InterPro) | 00620 | Pyruvate metabolism | InterPro 5.36-75.0 |
ECO:0000259
match to InterPro signature evidence used in automatic assertion |
|
| KEGG | bcj01230 | Biosynthesis of amino acids | 73.0+/03-31, Mar 15 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
Functional Predictions from Interpro
| Analysis | Accession | Description | Interpro Accession | Interpro Description | Amino Acid Start | Amino Acid Stop | E-value |
|---|---|---|---|---|---|---|---|
| Hamap | MF_00572 | 2-isopropylmalate synthase [leuA]. | IPR005668 | 2-isopropylmalate synthase | 7 | 549 | 53.429 |
| SUPERFAMILY | SSF89000 | 387 | 437 | 1.33E-15 | |||
| ProSitePatterns | PS00816 | Alpha-isopropylmalate and homocitrate synthases signature 2. | IPR002034 | Alpha-isopropylmalate/homocitrate synthase, conserved site | 242 | 255 | - |
| ProSitePatterns | PS00815 | Alpha-isopropylmalate and homocitrate synthases signature 1. | IPR002034 | Alpha-isopropylmalate/homocitrate synthase, conserved site | 39 | 55 | - |
| Gene3D | G3DSA:3.20.20.70 | IPR013785 | Aldolase-type TIM barrel | 10 | 375 | 1.1E-154 | |
| Gene3D | G3DSA:3.30.160.270 | 376 | 547 | 1.4E-53 | |||
| SUPERFAMILY | SSF110921 | IPR036230 | 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily | 443 | 547 | 1.2E-28 | |
| ProSiteProfiles | PS50991 | Pyruvate carboxyltransferase domain. | IPR000891 | Pyruvate carboxyltransferase | 32 | 306 | 19.09 |
| SMART | SM00917 | IPR013709 | 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain | 421 | 548 | 8.9E-27 | |
| TIGRFAM | TIGR00970 | leuA_yeast: 2-isopropylmalate synthase | IPR005668 | 2-isopropylmalate synthase | 6 | 547 | 1.2E-232 |
| Pfam | PF00682 | HMGL-like | IPR000891 | Pyruvate carboxyltransferase | 32 | 310 | 1.8E-74 |
| Pfam | PF08502 | LeuA allosteric (dimerisation) domain | IPR013709 | 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain | 421 | 547 | 5.9E-24 |
| CDD | cd07942 | DRE_TIM_LeuA | IPR039371 | LeuA, N-terminal catalytic TIM barrel domain | 31 | 314 | 0.0 |
| SUPERFAMILY | SSF51569 | 21 | 329 | 2.38E-99 |
Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.