Burkholderia cenocepacia J2315, BCAL0911 (miaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Molecular Function GO:0051536 iron-sulfur cluster binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF04055
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0035596 methylthiotransferase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PS51449
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0016740 transferase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_01864
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0003824 catalytic activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF04055
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006400 tRNA modification
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_01864
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0051539 4 iron, 4 sulfur cluster binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_01864
 ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
Pfam PF00919 Uncharacterized protein family UPF0004 IPR013848 Methylthiotransferase, N-terminal 4 104 2.1E-29
ProSitePatterns PS01278 Methylthiotransferase radical SAM domain signature. IPR020612 Methylthiotransferase, conserved site 151 171 -
SFLD SFLDG01082 B12-binding domain containing 32 360 7.2E-35
Gene3D G3DSA:3.80.30.20 IPR023404 Radical SAM, alpha/beta horseshoe 145 382 2.3E-82
TIGRFAM TIGR01574 miaB-methiolase: tRNA-i(6)A37 thiotransferase enzyme MiaB 4 446 8.4E-176
Hamap MF_01864 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase [miaB]. IPR006463 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB 3 447 75.442
SFLD SFLDF00273 (dimethylallyl)adenosine tRNA methylthiotransferase (MiaB-like) IPR006463 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB 2 446 0.0
ProSiteProfiles PS51449 Methylthiotransferase N-terminal domain profile. IPR013848 Methylthiotransferase, N-terminal 3 120 37.884
ProSiteProfiles PS50926 TRAM domain profile. IPR002792 TRAM domain 380 447 17.269
SFLD SFLDS00029 Radical SAM IPR007197 Radical SAM 2 446 0.0
Pfam PF01938 TRAM domain IPR002792 TRAM domain 380 446 4.0E-16
Gene3D G3DSA:3.40.50.12160 IPR038135 Methylthiotransferase, N-terminal domain superfamily 3 136 6.4E-33
SUPERFAMILY SSF102114 129 373 5.36E-61
Pfam PF04055 Radical SAM superfamily IPR007197 Radical SAM 151 326 9.0E-30
CDD cd01335 Radical_SAM 156 358 6.67332E-9
TIGRFAM TIGR00089 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family IPR005839 Methylthiotransferase 4 443 6.7E-147
SMART SM00729 IPR006638 Elp3/MiaB/NifB 147 369 1.4E-58

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.