Burkholderia thailandensis E264; ATCC 700388, BTH_II0863 (vgrG-5)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia thailandensis E264; ATCC 700388
Locus Tag
NCBI Locus TagBTH_RS04480
Replicon chromosome II
Genomic location 1009425 - 1012463 (- strand)
Transposon Mutants 3 transposon mutants in E264; ATCC 700388


RefSeq YP_439060.1
GI 83717090
Entrez 3844580
GI 83717090
NCBI Locus Tag BTH_RS04480
NCBI Old Locus Tag BTH_II0863
RefSeq WP_009896187.1
UniProtKB Acc Q2T6Y9


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) 20.03
Kyte-Doolittle Hydrophobicity Value -0.231
Molecular Weight (kDa) 108824.1
Isoelectric Point (pI) 9.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database BGD
Category T6SS-5
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes vgrG-5 mutation attenuated for virulence in RAW 264.7 macrophages
Reference 24452686
Source 2
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019353 (413 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0863
Search term: vgrG-5
Search term: VgrG-5

Transposon Insertions in E264; ATCC 700388

Type T23 (ISlacZ-PrhaBo-Tp/FRT ) insertion site
Mutant ID BTmutant: BT08190
Genomic Position 1011716
Position in ORF 2292(3039)
Reference 24194535
Type T23 (ISlacZ-PrhaBo-Tp/FRT ) insertion site
Mutant ID BTmutant: BT10056
Genomic Position 1010388
Position in ORF 964(3039)
Reference 24194535
Type T8 (ISlacZ hah-Tc ) insertion site
Mutant ID BTmutant: BT01948
Genomic Position 1010615
Position in ORF 1191(3039)
Reference 24194535

Transposon Insertions in Orthologs


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD.
PLoS Pathog. 2010 Aug 26;6(8)
PubMed ID: 20865170
VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence.
Schwarz S(1), Singh P, Robertson JD, LeRoux M, Skerrett SJ, Goodlett DR, West TE, Mougous JD.
Infect Immun. 2014 Apr;82(4):1445-52.
PubMed ID: 24452686