Burkholderia cenocepacia J2315, BCAM1946

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAM1946
NCBI Locus TagQU43_RS64075
Name
Replicon chromosome 2
Genomic location 2160520 - 2163693 (- strand)
CommentMember of rnd-9 operon encoding Resistance-Nodulation-Cell Division (RND) efflux pump RND-9
Comment Major RND-9 substrate is tobramycin, chlorhexidine (in biofilm grown cells)

Cross-References

RefSeq WP_006485132.1
GI 206563791
Entrez 6929577
GI 206563791
INSDC CAR55805.1
NCBI Locus Tag QU43_RS64075
NCBI Old Locus Tag BCAM1946
RefSeq WP_006485132.1,GeneID
UniParc UPI00017BA17D
UniProtKB Acc B4EMS9
UniProtKB ID B4EMS9_BURCJ
UniRef100 UniRef100_A0A0D1CER5
UniRef50 UniRef50_Q880Q4
UniRef90 UniRef90_B1K9Q0

Product

Feature Type CDS
Coding Frame 1
Product Name
RND-9 efflux system transporter protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 4.28
Kyte-Doolittle Hydrophobicity Value 0.351
Molecular Weight (kDa) 113414.6
Isoelectric Point (pI) 8.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
oqxB RND efflux pump conferring resistance to fluoroquinolone ARO:3003923 protein homolog model Strict 0.0 71.46 1.2.1

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001444 (2569 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAM1946

Human Homologs

References

Differential roles of RND efflux pumps in antimicrobial drug resistance of sessile and planktonic Burkholderia cenocepacia cells.
Buroni S, Matthijs N, Spadaro F, Van Acker H, Scoffone VC, Pasca MR, Riccardi G, Coenye T
Antimicrob Agents Chemother 2014 Dec;58(12):7424-9
PubMed ID: 25267676
Efflux pump-mediated drug resistance in Burkholderia.
Podnecky NL, Rhodes KA, Schweizer HP
Front Microbiol 2015;6:305
PubMed ID: 25926825