Burkholderia cenocepacia J2315, BCAM0581 (dfsA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
Locus Tag
NCBI Locus TagQU43_RS57295
Synonym: rpfF
Replicon chromosome 2
Genomic location 645965 - 646828 (- strand)


RefSeq WP_006488766.1
GI 206562441
Entrez 6928350
GI 206562441
INSDC CAR54439.1
NCBI Locus Tag QU43_RS57295
NCBI Old Locus Tag BCAM0581
RefSeq WP_006488766.1,GeneID
UniParc UPI00017B9A70
UniProtKB Acc B4EKM5
UniRef100 UniRef100_A0A095E9G9
UniRef50 UniRef50_E1SEP0
UniRef90 UniRef90_A0A071MPY0


Feature Type CDS
Coding Frame 1
Product Name
diffusible factor synthase A
Product Name Confidence: Class 1
Synonyms bifunctional crotonase homologue
Evidence for Translation
Charge (pH 7) -3.68
Kyte-Doolittle Hydrophobicity Value 0.006
Molecular Weight (kDa) 32000.4
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5FUS LYASE 01/29/16 Crystal structure of B. cenocepacia DfsA BURKHOLDERIA CENOCEPACIA 1.87 X-RAY DIFFRACTION 100.0
6DGN UNKNOWN FUNCTION 05/17/18 Cronobacter turicensis BDSF synthase RpfF in complex with the RpfR quorum-sensing receptor FI domain BURKHOLDERIA CENOCEPACIA PC184; CRONOBACTER TURICENSIS (STRAIN DSM 18703 / LMG 23827 / Z3032) 1.999 X-RAY DIFFRACTION 99.3

Virulence Evidence

Source 1
Database BGD
Category Quorum sensing system
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Candida albicans SC5314
Notes dfsA mutant has reduced ability to inhibit germ tube formation when cocultured with C. albicans
Reference 18049456

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 355 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG004647 (313 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BCAM0581
Search term: dfsA

Human Homologs


Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnover.
Deng Y, Schmid N, Wang C, Wang J, Pessi G, Wu D, Lee J, Aguilar C, Ahrens CH, Chang C, Song H, Eberl L, Zhang LH
Proc Natl Acad Sci U S A 2012 Sep 18;109(38):15479-84
PubMed ID: 22949660
A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition.
Boon C, Deng Y, Wang LH, He Y, Xu JL, Fan Y, Pan SQ, Zhang LH
ISME J 2008 Jan;2(1):27-36
PubMed ID: 18049456
The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.
Bi H, Christensen QH, Feng Y, Wang H, Cronan JE
Mol Microbiol 2012 Feb;83(4):840-55
PubMed ID: 22221091
Quorum sensing systems influence Burkholderia cenocepacia virulence.
Subramoni S, Sokol PA
Future Microbiol 2012 Dec;7(12):1373-87
PubMed ID: 23231487
The host plant metabolite glucose is the precursor of diffusible signal factor (DSF) family signals in Xanthomonas campestris.
Deng Y, Liu X, Wu J, Lee J, Chen S, Cheng Y, Zhang C, Zhang LH
Appl Environ Microbiol 2015 Apr;81(8):2861-8
PubMed ID: 25681189
The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter.
Suppiger A, Eshwar AK, Stephan R, Kaever V, Eberl L, Lehner A
Sci Rep 2016 Jan 4;6:18753
PubMed ID: 26725701