Burkholderia cenocepacia J2315, BCAM0581 (dfsA)

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAM0581
NCBI Locus Tag	QU43_RS57295
Name	dfsA Synonym: rpfF
Replicon	chromosome 2
Genomic location	645965 - 646828 (- strand)

Cross-References

RefSeq	WP_006488766.1
GI	206562441
Entrez	6928350
GI	206562441
INSDC	CAR54439.1
NCBI Locus Tag	QU43_RS57295
NCBI Old Locus Tag	BCAM0581
RefSeq	WP_006488766.1,GeneID
UniParc	UPI00017B9A70
UniProtKB Acc	B4EKM5
UniProtKB ID	B4EKM5_BURCJ
UniRef100	UniRef100_A0A095E9G9
UniRef50	UniRef50_E1SEP0
UniRef90	UniRef90_A0A071MPY0

Product

Feature Type	CDS
Coding Frame	1
Product Name	diffusible factor synthase A Product Name Confidence: Class 1
Synonyms	bifunctional crotonase homologue DfsA
Evidence for Translation
Charge (pH 7)	-3.68
Kyte-Doolittle Hydrophobicity Value	0.006
Molecular Weight (kDa)	32000.4
Isoelectric Point (pI)	5.97

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6DGN	UNKNOWN FUNCTION	05/17/18	Cronobacter turicensis BDSF synthase RpfF in complex with the RpfR quorum-sensing receptor FI domain	BURKHOLDERIA CENOCEPACIA PC184; CRONOBACTER TURICENSIS (STRAIN DSM 18703 / LMG 23827 / Z3032)	1.999	X-RAY DIFFRACTION	99.3
5FUS	LYASE	01/29/16	Crystal structure of B. cenocepacia DfsA	BURKHOLDERIA CENOCEPACIA	1.87	X-RAY DIFFRACTION	100.0

Virulence Evidence

Source 1
Database	BGD
Category	Quorum sensing system
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Candida albicans SC5314
Notes	dfsA mutant has reduced ability to inhibit germ tube formation when cocultured with C. albicans
Reference	18049456

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 355 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG004647 (313 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAM0581 Search term: dfsA Search term: diffusible factor synthase A

Human Homologs

References

Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnover. Deng Y, Schmid N, Wang C, Wang J, Pessi G, Wu D, Lee J, Aguilar C, Ahrens CH, Chang C, Song H, Eberl L, Zhang LH Proc Natl Acad Sci U S A 2012 Sep 18;109(38):15479-84 PubMed ID: 22949660

A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition. Boon C, Deng Y, Wang LH, He Y, Xu JL, Fan Y, Pan SQ, Zhang LH ISME J 2008 Jan;2(1):27-36 PubMed ID: 18049456

The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities. Bi H, Christensen QH, Feng Y, Wang H, Cronan JE Mol Microbiol 2012 Feb;83(4):840-55 PubMed ID: 22221091

Quorum sensing systems influence Burkholderia cenocepacia virulence. Subramoni S, Sokol PA Future Microbiol 2012 Dec;7(12):1373-87 PubMed ID: 23231487

The host plant metabolite glucose is the precursor of diffusible signal factor (DSF) family signals in Xanthomonas campestris. Deng Y, Liu X, Wu J, Lee J, Chen S, Cheng Y, Zhang C, Zhang LH Appl Environ Microbiol 2015 Apr;81(8):2861-8 PubMed ID: 25681189

The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter. Suppiger A, Eshwar AK, Stephan R, Kaever V, Eberl L, Lehner A Sci Rep 2016 Jan 4;6:18753 PubMed ID: 26725701