Burkholderia cenocepacia J2315, BCAL3111 (kdoO)

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAL3111
NCBI Locus Tag	QU43_RS52285
Name	kdoO Synonym: wbxY
Replicon	chromosome 1
Genomic location	3399895 - 3400779 (- strand)
Comment	Responsible for Ko formation with Kdo2-lipid A as a substrate

Cross-References

RefSeq	WP_006485119.1
GI	206561457
Entrez	6934235
GI	206561457
INSDC	CAR53435.1
NCBI Locus Tag	QU43_RS52285
NCBI Old Locus Tag	BCAL3111
RefSeq	WP_006485119.1,GeneID
UniParc	UPI00017B994F
UniProtKB Acc	B4ECD2
UniProtKB ID	B4ECD2_BURCJ
UniRef100	UniRef100_B4ECD2
UniRef50	UniRef50_Q0WGC8
UniRef90	UniRef90_B4ECD2

Product

Feature Type	CDS
Coding Frame	1
Product Name	Kdo 3-hydroxylase KdoO Product Name Confidence: Class 2
Synonyms	hypothetical protein
Evidence for Translation
Charge (pH 7)	3.59
Kyte-Doolittle Hydrophobicity Value	-0.222
Molecular Weight (kDa)	32740.3
Isoelectric Point (pI)	8.48

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 28 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG000751 (654 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAL3111 Search term: kdoO Search term: Kdo 3-hydroxylase KdoO

Human Homologs

References

Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis. Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA J Bacteriol 2005 Feb;187(4):1324-33 PubMed ID: 15687196

The LpxL acyltransferase is required for normal growth and penta-acylation of lipid A in Burkholderia cenocepacia. Fathy Mohamed Y, Hamad M, Ortega XP, Valvano MA Mol Microbiol 2017 Apr;104(1):144-162 PubMed ID: 28085228

Dioxygenases in Burkholderia ambifaria and Yersinia pestis that hydroxylate the outer Kdo unit of lipopolysaccharide. Chung HS, Raetz CR Proc Natl Acad Sci U S A 2011 Jan 11;108(2):510-5 PubMed ID: 21178073

Kdo hydroxylase is an inner core assembly enzyme in the Ko-containing lipopolysaccharide biosynthesis. Chung HS, Yang EG, Hwang D, Lee JE, Guan Z, Raetz CR Biochem Biophys Res Commun 2014 Sep 26;452(3):789-94 PubMed ID: 25204504