Burkholderia cenocepacia J2315, BCAL1536 (rpoN)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
Locus Tag
NCBI Locus TagQU43_RS44190
Replicon chromosome 1
Genomic location 1701800 - 1703191 (+ strand)


RefSeq WP_006483492.1
GI 206559902
Entrez 6931811
GI 206559902
INSDC CAR51835.1
NCBI Locus Tag QU43_RS44190
NCBI Old Locus Tag BCAL1536
RefSeq WP_006483492.1,GeneID
UniParc UPI00017B948D
UniProtKB Acc B4E832
UniRef100 UniRef100_A0A095DR90
UniRef50 UniRef50_I9BST1
UniRef90 UniRef90_C5AGX4


Feature Type CDS
Coding Frame 1
Product Name
alternative sigma factor RpoN
Product Name Confidence: Class 1
Synonyms putative sigma-54 related transcriptional regulatory protein
Evidence for Translation
Charge (pH 7) -10.21
Kyte-Doolittle Hydrophobicity Value -0.155
Molecular Weight (kDa) 51487.1
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Alternative Sigma factors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes rpoN mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146
Source 2
Database BGD
Category Alternative Sigma factors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes rpoN mutant cannot delay phagolysosomal fusion in mouse RAW 264.7 macrophages
Reference 18195023

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 427 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001356 (648 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BCAL1536
Search term: rpoN

Human Homologs


Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo.
Hunt TA, Kooi C, Sokol PA, Valvano MA
Infect Immun 2004 Jul;72(7):4010-22
PubMed ID: 15213146
Burkholderia cenocepacia requires the RpoN sigma factor for biofilm formation and intracellular trafficking within macrophages.
Saldías MS, Lamothe J, Wu R, Valvano MA
Infect Immun 2008 Mar;76(3):1059-67
PubMed ID: 18195023