Burkholderia cenocepacia MC0-3, Bcenmc03_6983 (prnA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Burkholderia cenocepacia MC0-3
GCF_000019505.1|latest
Locus Tag
Bcenmc03_6983
NCBI Locus TagBCENMC03_RS34850
Name
prnA
Replicon chromosome 3
Genomic location 1095394 - 1097010 (+ strand)

Cross-References

RefSeq WP_012337444.1
GI 170735465
Entrez 6125892
GI 170735465
NCBI Locus Tag BCENMC03_RS34850
NCBI Old Locus Tag Bcenmc03_6983
RefSeq WP_012337444.1
UniProtKB Acc B1K9Y4
UniProtKB ID B1K9Y4_BURCC

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophan halogenase
Product Name Confidence: Class 2
Synonyms tryptophan halogenase, PrnA
Evidence for Translation
Charge (pH 7) 0.11
Kyte-Doolittle Hydrophobicity Value -0.338
Molecular Weight (kDa) 61193.7
Isoelectric Point (pI) 7.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2ARD BIOSYNTHETIC PROTEIN 08/19/05 The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination PSEUDOMONAS FLUORESCENS 2.6 X-RAY DIFFRACTION 90.3
2JKC OXIDOREDUCTASE 08/26/08 Crystal Structure of E346D of Tryptophan 7-Halogenase (PrnA) PSEUDOMONAS FLUORESCENS 2.3 X-RAY DIFFRACTION 90.1
2APG BIOSYNTHETIC PROTEIN 08/16/05 The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination PSEUDOMONAS FLUORESCENS 1.9 X-RAY DIFFRACTION 90.3
2AQJ BIOSYNTHETIC PROTEIN 08/18/05 The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination PSEUDOMONAS FLUORESCENS 1.8 X-RAY DIFFRACTION 90.3
4Z44 OXIDOREDUCTASE 04/01/15 F454K Mutant of Tryptophan 7-halogenase PrnA PSEUDOMONAS FLUORESCENS 2.204 X-RAY DIFFRACTION 90.1
4Z43 OXIDOREDUCTASE 04/01/15 Crystal structure of Tryptophan 7-halogenase (PrnA) Mutant E450K PSEUDOMONAS FLUORESCENS 2.29 X-RAY DIFFRACTION 90.1
2AR8 BIOSYNTHETIC PROTEIN 08/19/05 The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination PSEUDOMONAS FLUORESCENS 2.2 X-RAY DIFFRACTION 90.3

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG015295 (318 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: Bcenmc03_6983
Search term: prnA

Human Homologs

References

Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of Burkholderia cepacia complex isolates.
Mendes R, Pizzirani-Kleiner AA, Araujo WL, Raaijmakers JM
Appl Environ Microbiol 2007 Nov;73(22):7259-67
PubMed ID: 17905875