Burkholderia thailandensis E264 ATCC 700388, BTH_II0863 (vgrG-5)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia thailandensis E264 ATCC 700388
Locus Tag
NCBI Locus TagBTH_RS04480
Replicon chromosome II
Genomic location 1009425 - 1012463 (- strand)
Transposon Mutants 3 transposon mutants in E264 ATCC 700388
CommentThis gene is part of cluster known as the type VI secretion system gene cluster tss-5 based on naming convention used for B. pseudomallei described in PMID:17660433, however unlike B. pseudomallei, the numbers following the vgrG and hcp gene match the cluster number (PMID:20865170). While other T6SSs were dispensible, T6SS-5 is required for virulence in a murine acute melioidosis model (PMID:20865170).


RefSeq YP_439060.1
GI 83717090
Entrez 3844580
GI 83717090
NCBI Locus Tag BTH_RS04480
NCBI Old Locus Tag BTH_II0863
RefSeq WP_009896187.1
UniProtKB Acc Q2T6Y9


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssI-5
Product Name Confidence: Class 2
Synonyms VgrG-5
Evidence for Translation
Charge (pH 7) 20.03
Kyte-Doolittle Hydrophobicity Value -0.231
Molecular Weight (kDa) 108824.1
Isoelectric Point (pI) 9.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Extracellular Class 2
sequence similarity evidence
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category T6SS-5
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes vgrG-5 mutation attenuated for virulence in RAW 264.7 macrophages
Reference 24452686
Source 2
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000250
sequence similarity evidence used in manual assertion
Host Organism
Annotation transferred from Burkholderia pseudomallei K96243
Reference 17984080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019353 (406 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BTH_II0863
Search term: vgrG-5

Human Homologs


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD
PLoS Pathog 2010 Aug 26;6(8):e1001068
PubMed ID: 20865170
VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence.
Schwarz S, Singh P, Robertson JD, LeRoux M, Skerrett SJ, Goodlett DR, West TE, Mougous JD
Infect Immun 2014 Apr;82(4):1445-52
PubMed ID: 24452686
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434