Burkholderia pseudomallei K96243, BPSS1495 (virA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Synonym: regA
Replicon chromosome 2
Genomic location 2038711 - 2040555 (- strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-5 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:21670170, PMID:22007185) and by the B. mallei literature (PMID:22892068, PMID:17555434).


RefSeq YP_111502.1
GI 53722517
Entrez 3096752
GI 53722517
INSDC CAH38968.1
NCBI Locus Tag BPSS1495
RefSeq YP_111502.1,GeneID
UniParc UPI000043BCFA
UniProtKB Acc Q63K70
UniRef100 UniRef100_Q63K70
UniRef50 UniRef50_Q63K70
UniRef90 UniRef90_Q63K70


Feature Type CDS
Coding Frame 1
Product Name
two-component regulatory system, sensor kinase VirA
Product Name Confidence: Class 1
Synonyms two-component regulator sensor kinase
Evidence for Translation
Charge (pH 7) 8.14
Kyte-Doolittle Hydrophobicity Value 0.309
Molecular Weight (kDa) 64369.0
Isoelectric Point (pI) 8.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 528 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001187 (1112 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1495
Search term: virA

Human Homologs


In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei.
Burtnick MN, Brett PJ, Harding SV, Ngugi SA, Ribot WJ, Chantratita N, Scorpio A, Milne TS, Dean RE, Fritz DL, Peacock SJ, Prior JL, Atkins TP, Deshazer D
Infect Immun 2011 Apr;79(4):1512-25
PubMed ID: 21300775
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434
Regulation of type VI secretion system during Burkholderia pseudomallei infection.
Chen Y, Wong J, Sun GW, Liu Y, Tan GY, Gan YH
Infect Immun 2011 Aug;79(8):3064-73
PubMed ID: 21670170
Host Cytosolic Glutathione Sensing by a Membrane Histidine Kinase Activates the Type VI Secretion System in an Intracellular Bacterium.
Wong J, Chen Y, Gan YH
Cell Host Microbe 2015 Jul 8;18(1):38-48
PubMed ID: 26094804
Bacterial Reductionism: Host Thiols Enhance Virulence.
Sperandio V
Cell Host Microbe 2015 Jul 8;18(1):7-8
PubMed ID: 26159714