Burkholderia cenocepacia H111, I35_RS02045 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia H111
GCF_000236215.2|latest
Locus Tag
I35_RS02045
NCBI Old Locus TagI35_0315%2CI35_0398
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Replicon chromosome 1
Genomic location 424802 - 426508 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq WP_006494382.1
GI 493540506
NCBI Locus Tag I35_RS02045
NCBI Old Locus Tag I35_0315%2CI35_0398
RefSeq WP_006494382.1

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms aldehyde dehydrogenase
Evidence for Translation
Charge (pH 7) -9.98
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 60516.7
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UJU OXIDOREDUCTASE 01/19/17 Crystal structure of NAD-dependent aldehyde dehydrogenase from Burkholderia multivorans BURKHOLDERIA MULTIVORANS (STRAIN ATCC 17616 / 249) 2.05 X-RAY DIFFRACTION 95.1

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: I35_RS02045
Search term: pacL
Search term: PacL

Human Homologs

References

Studies on the mechanism of ring hydrolysis in phenylacetate degradation: a metabolic branching point.
Teufel R, Gantert C, Voss M, Eisenreich W, Haehnel W, Fuchs G
J Biol Chem 2011 Apr 1;286(13):11021-34
PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ, Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST
J Bacteriol 2008 Nov;190(21):7209-18
PubMed ID: 18776009