Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS28540 (paaK1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
GCF_000333155.2|latest
Locus Tag
BURCENK562V_RS28540
NCBI Old Locus TagBURCENK562V_C6714
Name
paaK1
Replicon ctg7180000002941
Genomic location 515016 - 516314 (- strand)

Cross-References

RefSeq WP_006491104.1
GI 493537174
EC Number 6.2.1.30
GI 493537174
NCBI Locus Tag BURCENK562V_RS28540
NCBI Old Locus Tag BURCENK562V_C6714
RefSeq WP_006491104.1

Product

Feature Type CDS
Coding Frame 1
Product Name
PaaK1
Product Name Confidence: Class 1
Synonyms phenylacetate-CoA ligase
Evidence for Translation
Charge (pH 7) -6.12
Kyte-Doolittle Hydrophobicity Value -0.225
Molecular Weight (kDa) 47695.1
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2Y27 LIGASE 12/13/10 crystal structure of PaaK1 in complex with ATP from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA 1.6 X-RAY DIFFRACTION 100.0
2Y4N LIGASE 01/07/11 PaaK1 in complex with phenylacetyl adenylate BURKHOLDERIA CENOCEPACIA 1.92 X-RAY DIFFRACTION 100.0

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000429 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: paaK1
Search term: PaaK1

Human Homologs

References

Defining a structural and kinetic rationale for paralogous copies of phenylacetate-CoA ligases from the cystic fibrosis pathogen Burkholderia cenocepacia J2315.
Law A, Boulanger MJ
J Biol Chem 2011 Apr 29;286(17):15577-85
PubMed ID: 21388965