Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS29105 (wbiF)

Cytoplasmic Membrane
Outer Membrane

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
Locus Tag
NCBI Old Locus TagBURCENK562V_C5752
Replicon ctg7180000002940C
Genomic location 110286 - 111167 (+ strand)
CommentBelongs to the O antigen biosynthesis gene cluster


RefSeq WP_012492904.1
GI 501469459
GI 501469459
NCBI Locus Tag BURCENK562V_RS29105
NCBI Old Locus Tag BURCENK562V_C5752
RefSeq WP_012492904.1


Feature Type CDS
Coding Frame 1
Product Name
glycosyl transferase
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) 1.43
Kyte-Doolittle Hydrophobicity Value -0.074
Molecular Weight (kDa) 32622.7
Isoelectric Point (pI) 7.31

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Virulence Evidence

Source 1
Database BGD
Category Lipopolysaccharide O antigen
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes wbiF mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG006201 (533 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: wbiF

Human Homologs


A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo.
Loutet SA, Flannagan RS, Kooi C, Sokol PA, Valvano MA
J Bacteriol 2006 Mar;188(6):2073-80
PubMed ID: 16513737
Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis.
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA
J Bacteriol 2005 Feb;187(4):1324-33
PubMed ID: 15687196