Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS29090 (wbxE)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Burkholderia cenocepacia K56-2Valvano
GCF_000333155.2|latest |
| Locus Tag |
BURCENK562V_RS29090
|
| NCBI Old Locus Tag | BURCENK562V_C5749 |
| Name |
wbxE
|
| Replicon | ctg7180000002940C |
| Genomic location | 105970 - 107340 (+ strand) |
| Comment | Belongs to the O antigen biosynthesis gene cluster |
| Comment | B cenocepacia J2315, but not K56-2, is O-antigen deficient due to a transposon insertion in this gene. |
Cross-References
| RefSeq | WP_006493017.1 |
| GI | 493539127 |
| EC Number | 2.4.-.- |
| GI | 493539127 |
| NCBI Locus Tag | BURCENK562V_RS29090 |
| NCBI Old Locus Tag | BURCENK562V_C5749 |
| RefSeq | WP_006493017.1 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
WbxE
|
| Synonyms |
glycosyltransferase, group 1 family protein |
| Evidence for Translation | |
| Charge (pH 7) | 8.05 |
| Kyte-Doolittle Hydrophobicity Value | 0.076 |
| Molecular Weight (kDa) | 50801.4 |
| Isoelectric Point (pI) | 9.18 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
Virulence Evidence
| Source 1 | |
| Database | BGD |
| Category | Lipopolysaccharide O antigen |
| Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
| Host Organism | Rattus norvegicus |
| Notes | wbcE mutant attenuated for virulence in agar bead model of chronic lung infection |
| Reference | 15213146 |
Orthologs/Comparative Genomics
| Burkholderia Ortholog Group |
BG037146 (32 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: BURCENK562V_RS29090
Search term: wbxE
Search term: WbxE
|
Human Homologs
References
|
A complete lipopolysaccharide inner core oligosaccharide is required for resistance of Burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo.
Loutet SA, Flannagan RS, Kooi C, Sokol PA, Valvano MA
J Bacteriol 2006 Mar;188(6):2073-80
PubMed ID: 16513737
|
|
Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis.
Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA
J Bacteriol 2005 Feb;187(4):1324-33
PubMed ID: 15687196
|