Burkholderia pseudomallei 1026b, BP1026B_II1037 (penA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei 1026b - Assembly GCF_000260515.1
GCF_000260515.1|latest
Locus Tag
BP1026B_II1037
NCBI Locus TagBP1026B_RS23810
Name
penA
Replicon chromosome 2
Genomic location 1254633 - 1255520 (+ strand)
CommentB. multivorans PenA is an inhibitor-resistant carbapenemase, whereas the B. pseudomallei PenA is an extended spectrum beta-lactamase (PMID:27620956).

Cross-References

RefSeq
GI
Entrez 12832060
NCBI Locus Tag BP1026B_RS23810
NCBI Old Locus Tag BP1026B_II1037
RefSeq WP_004551611.1

Product

Feature Type CDS
Coding Frame 1
Product Name
class A beta-lactamase, PenA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 6.83
Kyte-Doolittle Hydrophobicity Value -0.068
Molecular Weight (kDa) 31315.3
Isoelectric Point (pI) 9.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6AFP HYDROLASE 08/08/18 Crystal structure of class A b-lactamase, PenL, variant Asn136Asp, from Burkholderia thailandensis, in complex with ceftazidime-like boronic acid BURKHOLDERIA THAILANDENSIS 1.398 X-RAY DIFFRACTION 90.4
3W4O HYDROLASE 01/10/13 Crystal structure of PenI beta-lactamase from Burkholderia pseudomallei at pH9.5 BURKHOLDERIA PSEUDOMALLEI 1.18 X-RAY DIFFRACTION 100.0
5GL9 HYDROLASE 07/10/16 Crystal structure of the class A beta-lactamase PenL BURKHOLDERIA THAILANDENSIS 1.5 X-RAY DIFFRACTION 90.8
3W4P HYDROLASE 01/10/13 Crystal structure of PenI beta-lactamase from Burkholderia pseudomallei at pH7.5 BURKHOLDERIA PSEUDOMALLEI 1.05 X-RAY DIFFRACTION 100.0
6AFO HYDROLASE 08/08/18 Crystal structure of class A b-lactamase, PenL, variant Asn136Asp, from Burkholderia thailandensis BURKHOLDERIA THAILANDENSIS 1.399 X-RAY DIFFRACTION 90.4
6AFM HYDROLASE 08/08/18 Crystal structure of class A b-lactamase, PenL, variant Cys69Tyr, from Burkholderia thailandensis BURKHOLDERIA THAILANDENSIS 1.3 X-RAY DIFFRACTION 90.4
6AFN HYDROLASE 08/08/18 Crystal structure of class A b-lactamase, PenL, variant Cys69Tyr, from Burkholderia thailandensis, in complex with ceftazidime-like boronic acid BURKHOLDERIA THAILANDENSIS 1.4 X-RAY DIFFRACTION 90.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 146 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG003909 (644 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BP1026B_II1037
Search term: penA

References

Pseudomonas pseudomallei resistance to beta-lactam antibiotics due to alterations in the chromosomally encoded beta-lactamase.
Godfrey AJ, Wong S, Dance DA, Chaowagul W, Bryan LE
Antimicrob Agents Chemother 1991 Aug;35(8):1635-40
PubMed ID: 1718214