Burkholderia pseudomallei K96243, BPSS1498 (tssD-5)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Synonym: hcp1
Replicon chromosome 2
Genomic location 2043052 - 2043561 (+ strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-5 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:21670170, PMID:22007185) and by the B. mallei literature (PMID:22892068, PMID:17555434).


RefSeq YP_111505.1
GI 53722520
Entrez 3095204
GI 53722520
INSDC CAH38971.1
NCBI Locus Tag BPSS1498
RefSeq YP_111505.1,GeneID
UniParc UPI000043BCFD
UniProtKB Acc Q63K67
UniRef100 UniRef100_G4XEK9
UniRef50 UniRef50_A5PM44
UniRef90 UniRef90_A5PM44


Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssD-5
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -3.56
Kyte-Doolittle Hydrophobicity Value -0.482
Molecular Weight (kDa) 18740.1
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Western blot evidence
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3WX6 UNKNOWN FUNCTION 07/17/14 Crystal structure of Type Six Secretion System protein BURKHOLDERIA PSEUDOMALLEI K96243 2.7 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Reference 17984080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019360 (317 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1498
Search term: tssD-5

Human Homologs


In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei.
Burtnick MN, Brett PJ, Harding SV, Ngugi SA, Ribot WJ, Chantratita N, Scorpio A, Milne TS, Dean RE, Fritz DL, Peacock SJ, Prior JL, Atkins TP, Deshazer D
Infect Immun 2011 Apr;79(4):1512-25
PubMed ID: 21300775
BPSS1504, a cluster 1 type VI secretion gene, is involved in intracellular survival and virulence of Burkholderia pseudomallei.
Hopf V, Göhler A, Eske-Pogodda K, Bast A, Steinmetz I, Breitbach K
Infect Immun 2014 May;82(5):2006-15
PubMed ID: 24595140
Regulation of type VI secretion system during Burkholderia pseudomallei infection.
Chen Y, Wong J, Sun GW, Liu Y, Tan GY, Gan YH
Infect Immun 2011 Aug;79(8):3064-73
PubMed ID: 21670170
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434