Burkholderia pseudomallei K96243, BPSS0962 (bcaA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
Replicon chromosome 2
Genomic location 1270105 - 1273500 (+ strand)


RefSeq YP_110970.1
GI 53721985
Entrez 3095427
GI 53721985
INSDC CAH38424.1
NCBI Locus Tag BPSS0962
RefSeq YP_110970.1,GeneID
UniParc UPI000043BAFA
UniProtKB Acc Q63LQ1
UniRef100 UniRef100_Q63LQ1
UniRef50 UniRef50_A1UV42
UniRef90 UniRef90_A1UV42


Feature Type CDS
Coding Frame 1
Product Name
Burkholderia classical autotransporter A, BcaA
Product Name Confidence: Class 1
Synonyms extracellular serine protease precursor
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724).
Charge (pH 7) 1.50
Kyte-Doolittle Hydrophobicity Value -0.109
Molecular Weight (kDa) 116008.8
Isoelectric Point (pI) 7.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Classical autotransporter
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes bcaA mutation attenuated for virulence in BALB/c mouse model of melioidosis.
Reference 25830295

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 136 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG003444 (802 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: BPSS0962
Search term: bcaA

Human Homologs


Systematic mutagenesis of genes encoding predicted autotransported proteins of Burkholderia pseudomallei identifies factors mediating virulence in mice, net intracellular replication and a novel protein conferring serum resistance.
Lazar Adler NR, Stevens MP, Dean RE, Saint RJ, Pankhania D, Prior JL, Atkins TP, Kessler B, Nithichanon A, Lertmemongkolchai G, Galyov EE
PLoS One 2015;10(4):e0121271
PubMed ID: 25830295
Characterization of BcaA, a putative classical autotransporter protein in Burkholderia pseudomallei.
Campos CG, Borst L, Cotter PA
Infect Immun 2013 Apr;81(4):1121-8
PubMed ID: 23340315