Burkholderia cenocepacia J2315, BCAM0854 (bceA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia cenocepacia J2315
GCF_000009485.1|latest
Locus Tag
BCAM0854
NCBI Locus TagQU43_RS58650
Name
bceA
Replicon chromosome 2
Genomic location 940538 - 942079 (- strand)
CommentMember of the bce-I gene cluster

Cross-References

RefSeq WP_012493179.1
GI 206562711
Entrez 6928609
GI 206562711
INSDC CAR54712.1
NCBI Locus Tag QU43_RS58650
NCBI Old Locus Tag BCAM0854
RefSeq WP_012493179.1,GeneID
UniParc UPI00017B99EE
UniProtKB Acc B4EMQ6
UniProtKB ID B4EMQ6_BURCJ
UniRef100 UniRef100_B4EMQ6
UniRef50 UniRef50_P24174
UniRef90 UniRef90_Q06XM8

Product

Feature Type CDS
Coding Frame 1
Product Name
bifunctional exopolysaccharide biosynthesis protein (phosphomannose isomerase and GDP-D-mannose pyrophosphorylase)
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.32
Kyte-Doolittle Hydrophobicity Value -0.144
Molecular Weight (kDa) 55715.7
Isoelectric Point (pI) 6.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Cepacian exopolysaccharide
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism
Notes
Reference 17184737

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 472 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG003312 (1226 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAM0854
Search term: bceA

Human Homologs

References

Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide.
Moreira LM, Videira PA, Sousa SA, Leitão JH, Cunha MV, Sá-Correia I
Biochem Biophys Res Commun 2003 Dec 12;312(2):323-33
PubMed ID: 14637140
Enzymes leading to the nucleotide sugar precursors for exopolysaccharide synthesis in Burkholderia cepacia.
Richau JA, Leitão JH, Sá-Correia I
Biochem Biophys Res Commun 2000 Sep 16;276(1):71-6
PubMed ID: 11006084
Functional analysis of the Burkholderia cenocepacia J2315 BceAJ protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities.
Sousa SA, Moreira LM, Leitão JH
Appl Microbiol Biotechnol 2008 Oct;80(6):1015-22
PubMed ID: 18668237
The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities.
Sousa SA, Moreira LM, Wopperer J, Eberl L, Sá-Correia I, Leitão JH
Biochem Biophys Res Commun 2007 Feb 2;353(1):200-6
PubMed ID: 17184737
Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions.
Ferreira AS, Leitão JH, Silva IN, Pinheiro PF, Sousa SA, Ramos CG, Moreira LM
Appl Environ Microbiol 2010 Jan;76(2):441-50
PubMed ID: 19948863