Burkholderia cenocepacia J2315, BCAL3119 (wbiI)

Gene Feature Overview

Strain	Burkholderia cenocepacia J2315 GCF_000009485.1|latest
Locus Tag	BCAL3119
NCBI Locus Tag	QU43_RS52325
Name	wbiI
Replicon	chromosome 1
Genomic location	3409126 - 3411009 (- strand)
Comment	Belongs to the O antigen biosynthesis gene cluster

Cross-References

RefSeq	WP_006485114.1
GI	206561465
Entrez	6934257
GI	206561465
INSDC	CAR53443.1
NCBI Locus Tag	QU43_RS52325
NCBI Old Locus Tag	BCAL3119
RefSeq	WP_006485114.1,GeneID
UniParc	UPI00017B9956
UniProtKB Acc	B4ECE0
UniProtKB ID	B4ECE0_BURCJ
UniRef100	UniRef100_B4ECE0
UniRef50	UniRef50_G2J9Y1
UniRef90	UniRef90_B4ECE0

Product

Feature Type	CDS
Coding Frame	1
Product Name	nucleotide sugar epimerase/dehydratase Product Name Confidence: Class 1
Synonyms	WbiI
Evidence for Translation
Charge (pH 7)	6.50
Kyte-Doolittle Hydrophobicity Value	0.171
Molecular Weight (kDa)	69230.0
Isoelectric Point (pI)	8.54

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	BGD
Category	Siderophore biosynthesis and uptake (ornibactin)
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Rattus norvegicus
Notes	wbiI mutant attenuated for virulence in agar bead model of chronic lung infection
Reference	15213146

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 464 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG000742 (649 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BCAL3119 Search term: wbiI Search term: nucleotide sugar epimerase/dehydratase

Human Homologs

References

Correlation of wbiI genotype, serotype, and isolate source within species of the Burkholderia cepacia complex. Vinion-Dubiel AD, Spilker T, Dean CR, Monteil H, LiPuma JJ, Goldberg JB J Clin Microbiol 2004 Sep;42(9):4121-6 PubMed ID: 15364998

Reconstitution of O-specific lipopolysaccharide expression in Burkholderia cenocepacia strain J2315, which is associated with transmissible infections in patients with cystic fibrosis. Ortega X, Hunt TA, Loutet S, Vinion-Dubiel AD, Datta A, Choudhury B, Goldberg JB, Carlson R, Valvano MA J Bacteriol 2005 Feb;187(4):1324-33 PubMed ID: 15687196