Burkholderia pseudomallei K96243, BPSS1525 (bopE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1525
Name
bopE
Replicon chromosome 2
Genomic location 2078020 - 2078805 (+ strand)
CommentThis gene is part of the type III secretion system T3SS-3 (bsa) gene locus.

Cross-References

RefSeq YP_111531.1
GI 53722546
Entrez 3096393
GI 53722546
INSDC CAH38998.1
NCBI Locus Tag BPSS1525
RefSeq YP_111531.1,GeneID
UniParc UPI000043BD17
UniProtKB Acc Q63K41
UniProtKB ID BOPE_BURPS
UniRef100 UniRef100_Q3JL30
UniRef50 UniRef50_Q2T704
UniRef90 UniRef90_Q2T704

Product

Feature Type CDS
Coding Frame 1
Product Name
guanine nucleotide exchange factor BopE (T3SS-3)
Product Name Confidence: Class 1
Synonyms G-nucleotide exchange factor
Evidence for Translation
Charge (pH 7) 8.14
Kyte-Doolittle Hydrophobicity Value -0.544
Molecular Weight (kDa) 28705.0
Isoelectric Point (pI) 9.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Western blot evidence used in manual assertion
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2JOL CELL INVASION, SIGNALING PROTEIN 03/14/07 Average NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI NOT SOLUTION NMR 100.0
2JOK CELL INVASION, SIGNALING PROTEIN 03/14/07 NMR structure of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI NOT SOLUTION NMR 100.0

Virulence Evidence

Source 1
Database VFDB : VF0428
Category T3SS-3
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080
Source 2
Database Victors : 1986
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes bopE mutant is attenuated in mice
Reference 16714590

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 3 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019825 (316 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1525
Search term: bopE

Human Homologs

References

Unraveling type III secretion systems in the highly versatile Burkholderia pseudomallei.
Sun GW, Gan YH
Trends Microbiol 2010 Dec;18(12):561-8
PubMed ID: 20951592
A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity.
Stevens MP, Friebel A, Taylor LA, Wood MW, Brown PJ, Hardt WD, Galyov EE
J Bacteriol 2003 Aug;185(16):4992-6
PubMed ID: 12897019
The guanine-nucleotide-exchange factor BopE from Burkholderia pseudomallei adopts a compact version of the Salmonella SopE/SopE2 fold and undergoes a closed-to-open conformational change upon interaction with Cdc42.
Upadhyay A, Wu HL, Williams C, Field T, Galyov EE, van den Elsen JM, Bagby S
Biochem J 2008 May 1;411(3):485-93
PubMed ID: 18052936
Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles.
Muangsombut V, Suparak S, Pumirat P, Damnin S, Vattanaviboon P, Thongboonkerd V, Korbsrisate S
Arch Microbiol 2008 Dec;190(6):623-31
PubMed ID: 18654761
Caspase-1-dependent and -independent cell death pathways in Burkholderia pseudomallei infection of macrophages.
Bast A, Krause K, Schmidt IH, Pudla M, Brakopp S, Hopf V, Breitbach K, Steinmetz I
PLoS Pathog 2014 Mar;10(3):e1003986
PubMed ID: 24626296
A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins.
Haque A, Chu K, Easton A, Stevens MP, Galyov EE, Atkins T, Titball R, Bancroft GJ
J Infect Dis 2006 Nov 1;194(9):1241-8
PubMed ID: 17041850
Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152