Burkholderia pseudomallei K96243, BPSS1503 (tssI-5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1503
Name
tssI-5
Synonym: vgrG-5
Replicon chromosome 2
Genomic location 2049856 - 2052879 (+ strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-5 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:21670170, PMID:22007185) and by the B. mallei literature (PMID:22892068, PMID:17555434).

Cross-References

RefSeq YP_111510.1
GI 53722525
Entrez 3095009
GI 53722525
NCBI Locus Tag BPSS1503
RefSeq YP_111510.1,GeneID
UniProtKB Acc Q63K62
UniProtKB ID Q63K62_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion protein TssI-5
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) 24.84
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 108229.7
Isoelectric Point (pI) 9.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0429
Category T6SS-1
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 109 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG019353 (406 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1503
Search term: tssI-5

Human Homologs

References

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence.
Schwarz S, Singh P, Robertson JD, LeRoux M, Skerrett SJ, Goodlett DR, West TE, Mougous JD
Infect Immun 2014 Apr;82(4):1445-52
PubMed ID: 24452686
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434