Burkholderia pseudomallei K96243, BPSS1400 (bpscU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1400
Name
bpscU
Synonym: sctU
Replicon chromosome 2
Genomic location 1913631 - 1914689 (+ strand)
CommentThis gene is part of the type III secretion system T3SS-1 locus found in B. pseudomallei but not B. mallei or B. thailandensis. B. pseudomallei T3SS-1 and T3SS-2, but not T3SS-3 contribute to pathogenicity in Solanum lycopersicum (tomato) but not human monocytic cell line THP-1. (PMID:20109238).

Cross-References

RefSeq YP_111412.1
GI 53722427
Entrez 3095565
GI 53722427
INSDC CAH38875.1
NCBI Locus Tag BPSS1400
RefSeq YP_111412.1,GeneID
UniParc UPI000043BCA4
UniProtKB Acc Q63KG0
UniProtKB ID Q63KG0_BURPS
UniRef100 UniRef100_I1WTY9
UniRef50 UniRef50_E5AVI5
UniRef90 UniRef90_I1WTY9

Product

Feature Type CDS
Coding Frame 1
Product Name
autoprotease, early/middle substrate switch BpscU (T3SS-1)
Product Name Confidence: Class 2
Synonyms type III secretion system protein HrcU
Evidence for Translation
Charge (pH 7) 5.39
Kyte-Doolittle Hydrophobicity Value 0.193
Molecular Weight (kDa) 38403.7
Isoelectric Point (pI) 9.39

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
T3SS
Note: This is based on PSORTb V3.0 secondary localization prediction
 Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 1982
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mesocricetus auratus
Notes sctU mutant is attenuated in hamsters
Reference 16714590

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 326 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG020880 (272 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1400
Search term: bpscU

Human Homologs

References

Identification of tomato plant as a novel host model for Burkholderia pseudomallei.
Lee YH, Chen Y, Ouyang X, Gan YH
BMC Microbiol 2010 Jan 29;10:28
PubMed ID: 20109238
Type III Secretion in the Melioidosis Pathogen <i>Burkholderia pseudomallei</i>.
Vander Broek CW, Stevens JM
Front Cell Infect Microbiol 2017;7:255
PubMed ID: 28664152
Role for the Burkholderia pseudomallei type three secretion system cluster 1 bpscN gene in virulence.
D'Cruze T, Gong L, Treerat P, Ramm G, Boyce JD, Prescott M, Adler B, Devenish RJ
Infect Immun 2011 Sep;79(9):3659-64
PubMed ID: 21768285
Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster.
Winstanley C, Hales BA, Hart CA
J Med Microbiol 1999 Jul;48(7):649-656
PubMed ID: 10403415
Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei.
Rainbow L, Hart CA, Winstanley C
J Med Microbiol 2002 May;51(5):374-384
PubMed ID: 11990489
Type III secretion system cluster 3 is required for maximal virulence of Burkholderia pseudomallei in a hamster infection model.
Warawa J, Woods DE
FEMS Microbiol Lett 2005 Jan 1;242(1):101-8
PubMed ID: 15621426