Burkholderia pseudomallei K96243, BPSS1385 (chbP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSS1385
Name
chbP
Synonym: cell cycle inhibiting factor Cif
Synonym: cif
Replicon chromosome 2
Genomic location 1893842 - 1894828 (- strand)
CommentCHBP contains a conserved catalytic triad (C156, H211, and Q231) that is important for cell cycle inhibition by deamindating Gln40 in ubiquitin (PMID: 20688984).

Cross-References

RefSeq YP_111397.1
GI 53722412
Entrez 3096526
GI 53722412
INSDC CAH38859.1
NCBI Locus Tag BPSS1385
RefSeq YP_111397.1,GeneID
UniParc UPI000043BC9B
UniProtKB Acc Q63KH5
UniProtKB ID Q63KH5_BURPS
UniRef100 UniRef100_I1WTX4
UniRef50 UniRef50_I1WTX4
UniRef90 UniRef90_I1WTX4

Product

Feature Type CDS
Coding Frame 1
Product Name
Cif homolog in Burkholderia pseudomallei (CHBP)
Product Name Confidence: Class 1
Synonyms ATP/GTP binding protein
Evidence for Translation
Charge (pH 7) -1.10
Kyte-Doolittle Hydrophobicity Value -0.356
Molecular Weight (kDa) 35818.3
Isoelectric Point (pI) 6.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Host Associated Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4HCP PROTEIN BINDING 10/01/12 crystal structure of Burkholderia pseudomallei effector protein chbp in complex with nedd8 BURKHOLDERIA PSEUDOMALLEI; HOMO SAPIENS 2.52 X-RAY DIFFRACTION 99.6
3EIT UNKNOWN FUNCTION 09/17/08 the 2.6 angstrom crystal structure of CHBP, the Cif Homologue from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.56 X-RAY DIFFRACTION 100.0
3EIR UNKNOWN FUNCTION 09/17/08 Crystal structure of CHBP, a Cif Homologue from Burkholderia pseudomallei BURKHOLDERIA PSEUDOMALLEI 2.101 X-RAY DIFFRACTION 100.0
3GQM UNKNOWN FUNCTION 03/24/09 Crystal structure of Cell Inhibiting Factor (Cif) from Burkholderia pseudomallei (CifBp) BURKHOLDERIA PSEUDOMALLEI 2.1 X-RAY DIFFRACTION 100.0
4HCN PROTEIN BINDING 09/30/12 Crystal structure of Burkholderia pseudomallei effector protein CHBP in complex with ubiquitin BURKHOLDERIA PSEUDOMALLEI; SACCHAROMYCES CEREVISIAE 2.6 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Pathogenic
Found only in pathogenic strains
Hits to this gene were found in 3 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG020859 (202 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSS1385
Search term: chbP

Human Homologs

References

Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei.
Pumirat P, Broek CV, Juntawieng N, Muangsombut V, Kiratisin P, Pattanapanyasat K, Stevens JM, Stevens MP, Korbsrisate S
PLoS One 2014;9(5):e96298
PubMed ID: 24809950
A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao R, Liu X, Huang N, Chen S, Liu L, Shao F
Proc Natl Acad Sci U S A 2009 Mar 10;106(10):3716-21
PubMed ID: 19225106
Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family.
Cui J, Yao Q, Li S, Ding X, Lu Q, Mao H, Liu L, Zheng N, Chen S, Shao F
Science 2010 Sep 3;329(5996):1215-8
PubMed ID: 20688984
Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif).
Ng MY, Wang M, Casey PJ, Gan YH, Hagen T
PLoS One 2017;12(2):e0171464
PubMed ID: 28166272