Burkholderia pseudomallei K96243, BPSL3101 (tssH-1)

Gene Feature Overview

Strain	Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1 GCF_000011545.1|latest
Locus Tag	BPSL3101
Name	tssH-1 Synonym: clpB
Replicon	chromosome 1
Genomic location	3704416 - 3707085 (- strand)
Comment	Gene is part of a cluster known as the type VI secretion system gene cluster tss-1 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:22007185)

Cross-References

RefSeq	YP_109696.1
GI	53720710
Entrez	3094210
GI	53720710
INSDC	CAH37112.1
NCBI Locus Tag	BPSL3101
RefSeq	YP_109696.1,GeneID
UniParc	UPI000043B617
UniProtKB Acc	Q63QC2
UniProtKB ID	Q63QC2_BURPS
UniRef100	UniRef100_I1WF20
UniRef50	UniRef50_Q2SUD4
UniRef90	UniRef90_Q2SUD4

Product

Feature Type	CDS
Coding Frame	1
Product Name	type VI secretion system protein TssH-1 Product Name Confidence: Class 2
Synonyms	protease associated ATPase ClpB
Evidence for Translation
Charge (pH 7)	-21.01
Kyte-Doolittle Hydrophobicity Value	-0.162
Molecular Weight (kDa)	96608.9
Isoelectric Point (pI)	5.33

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 597 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group	BG000372 (654 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: BPSL3101 Search term: tssH-1 Search term: type VI secretion system protein TssH-1

Human Homologs

Ensembl 97, assembly GRCh38.p12	ENSP00000443822 ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664] E-value: 3.9e-40 Percent Identity: 33.0
Ensembl 97, assembly GRCh38.p12	ENSP00000294053 ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664] E-value: 3.9e-40 Percent Identity: 33.0
Ensembl 97, assembly GRCh38.p12	ENSP00000441518 ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664] E-value: 3.9e-40 Percent Identity: 33.0
Ensembl 97, assembly GRCh38.p12	ENSP00000340385 ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664] E-value: 3.9e-40 Percent Identity: 33.0
Ensembl 97, assembly GRCh38.p12	ENSP00000407296 ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664] E-value: 3.9e-40 Percent Identity: 33.0

References

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Shalom G, Shaw JG, Thomas MS Microbiology 2007 Aug;153(Pt 8):2689-2699 PubMed ID: 17660433

Strategies for Intracellular Survival of Burkholderia pseudomallei. Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD Front Microbiol 2011;2:170 PubMed ID: 22007185

Type VI secretion is a major virulence determinant in Burkholderia mallei. Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D Mol Microbiol 2007 Jun;64(6):1466-85 PubMed ID: 17555434