Burkholderia pseudomallei K96243, BPSL3100 (tssA-1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL3100
Name
tssA-1
Replicon chromosome 1
Genomic location 3703205 - 3704326 (- strand)
CommentGene is part of a cluster known as the type VI secretion system gene cluster tss-1 used in much of the early P. pseudomallei literature (PMID:17660433) but later referred to as T6SS-1 cluster later (PMID:22007185)

Cross-References

RefSeq YP_109695.1
GI 53720709
Entrez 3093360
GI 53720709
INSDC CAH37111.1
NCBI Locus Tag BPSL3100
RefSeq YP_109695.1,GeneID
UniParc UPI000043B616
UniProtKB Acc Q63QC3
UniProtKB ID Q63QC3_BURPS
UniRef100 UniRef100_Q63QC3
UniRef50 UniRef50_A1UZK2
UniRef90 UniRef90_A1UZK2

Product

Feature Type CDS
Coding Frame 1
Product Name
type VI secretion system protein TssA-1
Product Name Confidence: Class 2
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -20.63
Kyte-Doolittle Hydrophobicity Value -0.346
Molecular Weight (kDa) 40936.3
Isoelectric Point (pI) 4.55

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6H8E TRANSPORT PROTEIN 08/02/18 Truncated derivative of the C-terminal domain of the TssA component of the type VI secretion system from Burkholderia cenocepacia BURKHOLDERIA CENOCEPACIA H111 2.35 X-RAY DIFFRACTION 90.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 76 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000373 (645 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3100
Search term: tssA-1

Human Homologs

References

In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Strategies for Intracellular Survival of Burkholderia pseudomallei.
Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD
Front Microbiol 2011;2:170
PubMed ID: 22007185
Type VI secretion is a major virulence determinant in Burkholderia mallei.
Schell MA, Ulrich RL, Ribot WJ, Brueggemann EE, Hines HB, Chen D, Lipscomb L, Kim HS, Mrázek J, Nierman WC, Deshazer D
Mol Microbiol 2007 Jun;64(6):1466-85
PubMed ID: 17555434