Burkholderia pseudomallei K96243, BPSL2270 (eno)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL2270
Name
eno
Replicon chromosome 1
Genomic location 2733734 - 2735017 (- strand)

Cross-References

RefSeq YP_108866.1
GI 53719880
Entrez 3091594
GI 53719880
INSDC CAH36273.1
NCBI Locus Tag BPSL2270
RefSeq YP_108866.1,GeneID
UniParc UPI000043B301
UniProtKB Acc Q63SQ0
UniProtKB ID ENO_BURPS
UniRef100 UniRef100_A3ML77
UniRef50 UniRef50_Q5XD01
UniRef90 UniRef90_B2U9C3

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphopyruvate hydratase
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (45.4 kDa, pI 4.78, PMID:21960737)
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (45.4 kDa, pI 4.76, PMID:21960737)
Charge (pH 7) -18.65
Kyte-Doolittle Hydrophobicity Value -0.099
Molecular Weight (kDa) 45682.2
Isoelectric Point (pI) 4.55

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 663 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG002000 (654 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL2270
Search term: eno

Human Homologs

Ensembl 97, assembly GRCh38.p12
enolase 2 [Source:HGNC Symbol;Acc:HGNC:3353]
E-value: 1.9e-112
Percent Identity: 52.2
Ensembl 97, assembly GRCh38.p12
enolase 2 [Source:HGNC Symbol;Acc:HGNC:3353]
E-value: 1.9e-112
Percent Identity: 52.2
Ensembl 97, assembly GRCh38.p12
enolase 2 [Source:HGNC Symbol;Acc:HGNC:3353]
E-value: 1.9e-112
Percent Identity: 52.2

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268