Burkholderia pseudomallei K96243, BPSL1705 (boaB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL1705
Name
boaB
Replicon chromosome 1
Genomic location 1982643 - 1987463 (+ strand)

Cross-References

RefSeq YP_108306.1
GI 53719320
Entrez 3094881
GI 53719320
NCBI Locus Tag BPSL1705
RefSeq YP_108306.1,GeneID
UniProtKB Acc Q63UB0
UniProtKB ID Q63UB0_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
BoaB
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -19.90
Kyte-Doolittle Hydrophobicity Value 0.154
Molecular Weight (kDa) 148819.0
Isoelectric Point (pI) 4.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database BGD
Category Trimeric autotransporter
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes boaB mutation has reduced net intracellular replication in J774.2 murine macrophage-like cells
Reference 25830295
Source 2
Database VFDB : VF0435
Category Burkholderia oligomeric coiled-coil adhesin B
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism
Notes
Reference 17984080
Source 3
Database Victors : 2016
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes boaB (BPSL1705) mutant has reduced attachment to respiratory cells, and a boaA boaB double mutant was attenuated in murine macrophages
Reference 16988221

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 81 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG002926 (1913 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL1705
Search term: boaB
Search term: BoaB

Human Homologs

References

Acid peptic disease.
J Assoc Physicians India 1990 Sep;38 Suppl 1:683-751
PubMed ID: 2092018
Systematic mutagenesis of genes encoding predicted autotransported proteins of Burkholderia pseudomallei identifies factors mediating virulence in mice, net intracellular replication and a novel protein conferring serum resistance.
Lazar Adler NR, Stevens MP, Dean RE, Saint RJ, Pankhania D, Prior JL, Atkins TP, Kessler B, Nithichanon A, Lertmemongkolchai G, Galyov EE
PLoS One 2015;10(4):e0121271
PubMed ID: 25830295