Burkholderia pseudomallei K96243, BPSL1510 (ndk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL1510
Name
ndk
Replicon chromosome 1
Genomic location 1748592 - 1749017 (+ strand)

Cross-References

RefSeq YP_108130.1
GI 53719144
Entrez 3091685
GI 53719144
INSDC CAH35511.1
NCBI Locus Tag BPSL1510
RefSeq YP_108130.1,GeneID
UniParc UPI000043B027
UniProtKB Acc Q63UT6
UniProtKB ID NDK_BURPS
UniRef100 UniRef100_A3MK78
UniRef50 UniRef50_Q5WGT0
UniRef90 UniRef90_Q2SWE7

Product

Feature Type CDS
Coding Frame 1
Product Name
nucleoside diphosphate kinase
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain 844 using MALDI-TOF/MS analysis (16.0 kDa, pI 5.88, PMID:21960737)
Charge (pH 7) -2.29
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 15561.6
Isoelectric Point (pI) 5.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4EK2 TRANSFERASE 04/09/12 The structure of nucleoside diphosphate kinase (NDK) from Burkholderia thailandensis bound to deoxyadenosine monophosphate BURKHOLDERIA THAILANDENSIS 2.0 X-RAY DIFFRACTION 97.9
4DUT TRANSFERASE 02/22/12 The structure of nucleoside diphosphate kinase (NDK) from Burkholderia thailandensis BURKHOLDERIA THAILANDENSIS 2.5 X-RAY DIFFRACTION 97.9
4HR2 TRANSFERASE 10/26/12 Structure of nucleoside diphosphate kinase (NDK) from Burkholderia thailandensis bound to ADP BURKHOLDERIA THAILANDENSIS 1.95 X-RAY DIFFRACTION 97.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001652 (653 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL1510
Search term: ndk

Human Homologs

Ensembl 97, assembly GRCh38.p12
NME/NM23 nucleoside diphosphate kinase 3 [Source:HGNC Symbol;Acc:HGNC:7851]
E-value: 9.7e-26
Percent Identity: 43.9

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268