Burkholderia lata 383, BCEP18194_RS08230 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia lata 383
Locus Tag
BCEP18194_RS08230
NCBI Old Locus TagBcep18194_A3630
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Replicon chromosome 1
Genomic location 504183 - 505889 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq WP_011350826.1
GI 499670092
NCBI Locus Tag BCEP18194_RS08230
NCBI Old Locus Tag Bcep18194_A3630
RefSeq WP_011350826.1

Product

Feature Type CDS
Coding Frame NA
Product Name
PacL
Product Name Confidence: Class 2
Synonyms aldehyde dehydrogenase
Evidence for Translation
Charge (pH 7) -10.98
Kyte-Doolittle Hydrophobicity Value -0.060
Molecular Weight (kDa) 60595.8
Isoelectric Point (pI) 5.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UJU OXIDOREDUCTASE 01/19/17 Crystal structure of NAD-dependent aldehyde dehydrogenase from Burkholderia multivorans BURKHOLDERIA MULTIVORANS (STRAIN ATCC 17616 / 249) 2.05 X-RAY DIFFRACTION 95.1

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCEP18194_RS08230
Search term: pacL
Search term: PacL

References

PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009