Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS28560 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
Locus Tag
BURCENK562V_RS28560
NCBI Old Locus TagBURCENK562V_C6718
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Synonym: paaZ
Replicon ctg7180000002941
Genomic location 518934 - 520640 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq WP_006491101.1
GI 493537171
GI 493537171
NCBI Locus Tag BURCENK562V_RS28560
NCBI Old Locus Tag BURCENK562V_C6718
RefSeq WP_006491101.1

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms aldehyde dehydrogenase
Evidence for Translation
Charge (pH 7) -9.98
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 60502.6
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UJU OXIDOREDUCTASE 01/19/17 Crystal structure of NAD-dependent aldehyde dehydrogenase from Burkholderia multivorans BURKHOLDERIA MULTIVORANS (STRAIN ATCC 17616 / 249) 2.05 X-RAY DIFFRACTION 94.9

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: pacL
Search term: PacL

References

A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009
PubMed ID: 21296885