Burkholderia mallei ATCC 23344, BMAA0543 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia mallei ATCC 23344
GCF_000011705.1|latest
Locus Tag
BMAA0543
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Replicon chromosome 2
Genomic location 551783 - 553489 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq YP_105311.1
GI 53717328
NCBI Locus Tag BMAA0543
RefSeq YP_105311.1,GeneID

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms aldehyde dehydrogenase
Evidence for Translation
Charge (pH 7) -4.77
Kyte-Doolittle Hydrophobicity Value -0.090
Molecular Weight (kDa) 60632.9
Isoelectric Point (pI) 6.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Pathogen Association Analysis

Results Analysis not done

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BMAA0543
Search term: pacL
Search term: PacL

Human Homologs

References

Studies on the mechanism of ring hydrolysis in phenylacetate degradation: a metabolic branching point.
Teufel R, Gantert C, Voss M, Eisenreich W, Haehnel W, Fuchs G
J Biol Chem 2011 Apr 1;286(13):11021-34
PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ, Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST
J Bacteriol 2008 Nov;190(21):7209-18
PubMed ID: 18776009